catalog numbers 4625 Search Results


hydrochloric acid carl roth 4625.1  (Carl Roth GmbH)
90
Carl Roth GmbH hydrochloric acid carl roth 4625.1
Hydrochloric Acid Carl Roth 4625.1, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalog+numbers+4625/hcl++carl+roth++de+/10__21769_slash_bioprotoc__2920-40-0-2
Average 90 stars, based on 1 article reviews
hydrochloric acid carl roth 4625.1 - by Bioz Stars, 2026-10
90/100 stars
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anti a20 antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti a20 antibody
Figure 3. Effects of thiamet G on <t>A20-mediated</t> inhibition of IκBα degradation and p65 phosphorylation in the presence of TNF-α (tumor necrosis factor-α). Vascular smooth muscle cells (VSMCs) were infected with an adenovirus carrying A20 (Ad-A20) or an empty vec- tor (Ad-null) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 30 min. Levels of IκBα and phosphorylated p65 were determined by Western blotting analysis. GAPDH served as an internal control. Results are expressed as mean±SEM, n=6. *P<0.05. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.
Anti A20 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalog+numbers+4625/A20+Antibody/10__1161_slash_atvbaha__117__310468-49-34-20
Average 94 stars, based on 1 article reviews
anti a20 antibody - by Bioz Stars, 2026-10
94/100 stars
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hydrochloric acid  (Carl Roth GmbH)
90
Carl Roth GmbH hydrochloric acid
Figure 3. Effects of thiamet G on <t>A20-mediated</t> inhibition of IκBα degradation and p65 phosphorylation in the presence of TNF-α (tumor necrosis factor-α). Vascular smooth muscle cells (VSMCs) were infected with an adenovirus carrying A20 (Ad-A20) or an empty vec- tor (Ad-null) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 30 min. Levels of IκBα and phosphorylated p65 were determined by Western blotting analysis. GAPDH served as an internal control. Results are expressed as mean±SEM, n=6. *P<0.05. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.
Hydrochloric Acid, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalog+numbers+4625/hydrochloric+acid/pmc06046211-37-80-82
Average 90 stars, based on 1 article reviews
hydrochloric acid - by Bioz Stars, 2026-10
90/100 stars
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hydrochloric acid fuming, 37% (hcl)  (Carl Roth GmbH)
90
Carl Roth GmbH hydrochloric acid fuming, 37% (hcl)
Figure 3. Effects of thiamet G on <t>A20-mediated</t> inhibition of IκBα degradation and p65 phosphorylation in the presence of TNF-α (tumor necrosis factor-α). Vascular smooth muscle cells (VSMCs) were infected with an adenovirus carrying A20 (Ad-A20) or an empty vec- tor (Ad-null) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 30 min. Levels of IκBα and phosphorylated p65 were determined by Western blotting analysis. GAPDH served as an internal control. Results are expressed as mean±SEM, n=6. *P<0.05. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.
Hydrochloric Acid Fuming, 37% (Hcl), supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalog+numbers+4625/hydrochloric+acid++25+/10__21769_slash_bioprotoc__2355-52-4-5
Average 90 stars, based on 1 article reviews
hydrochloric acid fuming, 37% (hcl) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Effects of thiamet G on A20-mediated inhibition of IκBα degradation and p65 phosphorylation in the presence of TNF-α (tumor necrosis factor-α). Vascular smooth muscle cells (VSMCs) were infected with an adenovirus carrying A20 (Ad-A20) or an empty vec- tor (Ad-null) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 30 min. Levels of IκBα and phosphorylated p65 were determined by Western blotting analysis. GAPDH served as an internal control. Results are expressed as mean±SEM, n=6. *P<0.05. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: O-Linked β-N-Acetylglucosamine Modification of A20 Enhances the Inhibition of NF-κB (Nuclear Factor-κB) Activation and Elicits Vascular Protection After Acute Endoluminal Arterial Injury

doi: 10.1161/atvbaha.117.310468

Figure Lengend Snippet: Figure 3. Effects of thiamet G on A20-mediated inhibition of IκBα degradation and p65 phosphorylation in the presence of TNF-α (tumor necrosis factor-α). Vascular smooth muscle cells (VSMCs) were infected with an adenovirus carrying A20 (Ad-A20) or an empty vec- tor (Ad-null) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 30 min. Levels of IκBα and phosphorylated p65 were determined by Western blotting analysis. GAPDH served as an internal control. Results are expressed as mean±SEM, n=6. *P<0.05. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.

Article Snippet: After cell lysis, 600 mg of total protein per sample were precleaned with normal rat/ mouse IgG and proteinA/G-plus beads (Santa Cruz) and then immunoprecipitated with anti-O-GlcNAc (clone 18B10.C7; catalog number 05-1244; Millipore) or anti-A20 antibody (catalog number 4625; Cell Signaling Tech), respectively, at 4°C overnight.

Techniques: Inhibition, Phospho-proteomics, Infection, Western Blot, Control, Comparison

Figure 6. Silencing A20 abolished the inhibitory effect of thiamet G on TNF-α (tumor necrosis factor-α)–induced migration and prolifera- tion in vascular smooth muscle cells (VSMCs). VSMCs were infected with an adenovirus carrying A20 (Ad-A20), A20 shRNA (Ad-A20 shRNA), or scramble shRNA (Ad-scramble shRNA) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 12 h (A and B) or 24 h. C, VSMCs migration was determined by the Transwell Boyden chamber method. Proliferation of VSMCs was measured using a CCK-8 kit. Results are expressed as mean±SEM, n=8. *P<0.05. NS indicates no significance. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: O-Linked β-N-Acetylglucosamine Modification of A20 Enhances the Inhibition of NF-κB (Nuclear Factor-κB) Activation and Elicits Vascular Protection After Acute Endoluminal Arterial Injury

doi: 10.1161/atvbaha.117.310468

Figure Lengend Snippet: Figure 6. Silencing A20 abolished the inhibitory effect of thiamet G on TNF-α (tumor necrosis factor-α)–induced migration and prolifera- tion in vascular smooth muscle cells (VSMCs). VSMCs were infected with an adenovirus carrying A20 (Ad-A20), A20 shRNA (Ad-A20 shRNA), or scramble shRNA (Ad-scramble shRNA) for 3 d. Cells were pretreated with vehicle or thiamet G for 2 h and then stimulated with TNF-α for 12 h (A and B) or 24 h. C, VSMCs migration was determined by the Transwell Boyden chamber method. Proliferation of VSMCs was measured using a CCK-8 kit. Results are expressed as mean±SEM, n=8. *P<0.05. NS indicates no significance. Statistical analysis: 1-way ANOVA followed by Bonferroni multiple comparison tests.

Article Snippet: After cell lysis, 600 mg of total protein per sample were precleaned with normal rat/ mouse IgG and proteinA/G-plus beads (Santa Cruz) and then immunoprecipitated with anti-O-GlcNAc (clone 18B10.C7; catalog number 05-1244; Millipore) or anti-A20 antibody (catalog number 4625; Cell Signaling Tech), respectively, at 4°C overnight.

Techniques: Migration, Infection, shRNA, CCK-8 Assay, Comparison